Cloning and characterization of a wheat vacuolar cation/proton antiporter and pyrophosphatase proton pump.
Identifieur interne : 000021 ( Main/Exploration ); précédent : 000020; suivant : 000022Cloning and characterization of a wheat vacuolar cation/proton antiporter and pyrophosphatase proton pump.
Auteurs : Faïçal Brini [Tunisie] ; Roberto A. Gaxiola ; Gerald A. Berkowitz ; Khaled MasmoudiSource :
- Plant physiology and biochemistry : PPB [ 0981-9428 ] ; 2005.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN des plantes (génétique), Antiport des ions sodium-hydrogène (génétique), Antiport des ions sodium-hydrogène (métabolisme), Chlorure de sodium (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Expression des gènes (MeSH), Gènes de plante (MeSH), Inorganic Pyrophosphatase (génétique), Inorganic Pyrophosphatase (métabolisme), Mutation (MeSH), Phylogenèse (MeSH), Plasmides (génétique), Pression osmotique (MeSH), Protéines végétales (génétique), Protéines végétales (métabolisme), Saccharomyces cerevisiae (génétique), Similitude de séquences d'acides aminés (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Transporteurs de cations (génétique), Transporteurs de cations (métabolisme), Triticum (génétique), Triticum (métabolisme), Vacuolar Proton-Translocating ATPases (génétique), Vacuolar Proton-Translocating ATPases (métabolisme).
- MESH :
- génétique : ADN complémentaire, ADN des plantes, Antiport des ions sodium-hydrogène, Inorganic Pyrophosphatase, Plasmides, Protéines végétales, Saccharomyces cerevisiae, Transporteurs de cations, Triticum, Vacuolar Proton-Translocating ATPases.
- métabolisme : Antiport des ions sodium-hydrogène, Inorganic Pyrophosphatase, Protéines végétales, Transporteurs de cations, Triticum, Vacuolar Proton-Translocating ATPases.
- Chlorure de sodium, Clonage moléculaire, Données de séquences moléculaires, Expression des gènes, Gènes de plante, Mutation, Phylogenèse, Pression osmotique, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Cation Transport Proteins (genetics), Cation Transport Proteins (metabolism), Cloning, Molecular (MeSH), DNA, Complementary (genetics), DNA, Plant (genetics), Gene Expression (MeSH), Genes, Plant (MeSH), Inorganic Pyrophosphatase (genetics), Inorganic Pyrophosphatase (metabolism), Molecular Sequence Data (MeSH), Mutation (MeSH), Osmotic Pressure (MeSH), Phylogeny (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plasmids (genetics), Saccharomyces cerevisiae (genetics), Sequence Homology, Amino Acid (MeSH), Sodium Chloride (MeSH), Sodium-Hydrogen Exchangers (genetics), Sodium-Hydrogen Exchangers (metabolism), Triticum (genetics), Triticum (metabolism), Vacuolar Proton-Translocating ATPases (genetics), Vacuolar Proton-Translocating ATPases (metabolism).
- MESH :
- chemical , genetics : Cation Transport Proteins, DNA, Complementary, DNA, Plant, Inorganic Pyrophosphatase, Plant Proteins, Sodium-Hydrogen Exchangers, Vacuolar Proton-Translocating ATPases.
- chemical , metabolism : Cation Transport Proteins, Inorganic Pyrophosphatase, Plant Proteins, Sodium-Hydrogen Exchangers, Vacuolar Proton-Translocating ATPases.
- genetics : Plasmids, Saccharomyces cerevisiae, Triticum.
- metabolism : Triticum.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Gene Expression, Genes, Plant, Molecular Sequence Data, Mutation, Osmotic Pressure, Phylogeny, Sequence Homology, Amino Acid, Sodium Chloride.
Abstract
Sodium at high millimolar levels in the cytoplasm is toxic to plant and yeast cells. Sequestration of Na+ ions into the vacuole through the action of tonoplast proton pumps (an H+ -ATPase in the case of yeast, and either a H+ -pyrophosphatase (H+ -PPase) or H+ -ATPase in the case of plants) and a Na+/H+ antiporter is one mechanism that confers salt tolerance to these organisms. The cloning and characterization of genes encoding these tonoplast transport proteins from crop plants may contribute to our understanding of how to enhance crop plant response to saline stress. We cloned wheat orthologs of the Arabidopsis genes AtNHX1 and AVP1 using the polymerase chain reaction and primers corresponding to conserved regions of the respective coding sequences, and a wheat cDNA library as template. The wheat NHX cDNA cloned by this approach was a variant of the previously reported TNHX1 gene. The vacuolar H+ -PPase pump we cloned (TVP1) is the first member of this gene family cloned from wheat; it is deduced translation product is homologous to proteins encoded by genes in barley, rice, and Arabidopsis. Function of TNHX1 as a cation/proton antiporter was demonstrated using the nhx1 yeast mutant. TNHX1 was capable of suppressing the hyg sensitivity of nhx1. Functional characterization of the wheat H+ -PPase TVP1 was demonstrated using the yeast ena1 (plasma membrane Na+ -efflux transporter) mutant. Expression of TVP1 in ena1 suppressed its Na+ hypersensitivity. Expression analysis of salt-stressed wheat plants showed substantial up-regulation of TNHX1 transcript levels as compared to control plants, while transcript accumulation for TVP1 was not greatly affected by exposure of plants to salt stress.
DOI: 10.1016/j.plaphy.2005.02.010
PubMed: 15907686
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000803
- to stream PubMed, to step Curation: 000799
- to stream PubMed, to step Checkpoint: 000799
- to stream Main, to step Merge: 000021
- to stream Main, to step Curation: 000021
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cloning and characterization of a wheat vacuolar cation/proton antiporter and pyrophosphatase proton pump.</title><author><name sortKey="Brini, Faical" sort="Brini, Faical" uniqKey="Brini F" first="Faïçal" last="Brini">Faïçal Brini</name><affiliation wicri:level="1"><nlm:affiliation>Plant Molecular Genetics Unit, Center of Biotechnology of Sfax, B.P'K', 3038 Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Plant Molecular Genetics Unit, Center of Biotechnology of Sfax, B.P'K', 3038 Sfax</wicri:regionArea><wicri:noRegion>3038 Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Gaxiola, Roberto A" sort="Gaxiola, Roberto A" uniqKey="Gaxiola R" first="Roberto A" last="Gaxiola">Roberto A. Gaxiola</name></author><author><name sortKey="Berkowitz, Gerald A" sort="Berkowitz, Gerald A" uniqKey="Berkowitz G" first="Gerald A" last="Berkowitz">Gerald A. Berkowitz</name></author><author><name sortKey="Masmoudi, Khaled" sort="Masmoudi, Khaled" uniqKey="Masmoudi K" first="Khaled" last="Masmoudi">Khaled Masmoudi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15907686</idno><idno type="pmid">15907686</idno><idno type="doi">10.1016/j.plaphy.2005.02.010</idno><idno type="wicri:Area/PubMed/Corpus">000803</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000803</idno><idno type="wicri:Area/PubMed/Curation">000799</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000799</idno><idno type="wicri:Area/PubMed/Checkpoint">000799</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000799</idno><idno type="wicri:Area/Main/Merge">000021</idno><idno type="wicri:Area/Main/Curation">000021</idno><idno type="wicri:Area/Main/Exploration">000021</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning and characterization of a wheat vacuolar cation/proton antiporter and pyrophosphatase proton pump.</title><author><name sortKey="Brini, Faical" sort="Brini, Faical" uniqKey="Brini F" first="Faïçal" last="Brini">Faïçal Brini</name><affiliation wicri:level="1"><nlm:affiliation>Plant Molecular Genetics Unit, Center of Biotechnology of Sfax, B.P'K', 3038 Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Plant Molecular Genetics Unit, Center of Biotechnology of Sfax, B.P'K', 3038 Sfax</wicri:regionArea><wicri:noRegion>3038 Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Gaxiola, Roberto A" sort="Gaxiola, Roberto A" uniqKey="Gaxiola R" first="Roberto A" last="Gaxiola">Roberto A. Gaxiola</name></author><author><name sortKey="Berkowitz, Gerald A" sort="Berkowitz, Gerald A" uniqKey="Berkowitz G" first="Gerald A" last="Berkowitz">Gerald A. Berkowitz</name></author><author><name sortKey="Masmoudi, Khaled" sort="Masmoudi, Khaled" uniqKey="Masmoudi K" first="Khaled" last="Masmoudi">Khaled Masmoudi</name></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="ISSN">0981-9428</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Cation Transport Proteins (genetics)</term><term>Cation Transport Proteins (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Complementary (genetics)</term><term>DNA, Plant (genetics)</term><term>Gene Expression (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Inorganic Pyrophosphatase (genetics)</term><term>Inorganic Pyrophosphatase (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Mutation (MeSH)</term><term>Osmotic Pressure (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plasmids (genetics)</term><term>Saccharomyces cerevisiae (genetics)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Sodium Chloride (MeSH)</term><term>Sodium-Hydrogen Exchangers (genetics)</term><term>Sodium-Hydrogen Exchangers (metabolism)</term><term>Triticum (genetics)</term><term>Triticum (metabolism)</term><term>Vacuolar Proton-Translocating ATPases (genetics)</term><term>Vacuolar Proton-Translocating ATPases (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ADN des plantes (génétique)</term><term>Antiport des ions sodium-hydrogène (génétique)</term><term>Antiport des ions sodium-hydrogène (métabolisme)</term><term>Chlorure de sodium (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Inorganic Pyrophosphatase (génétique)</term><term>Inorganic Pyrophosphatase (métabolisme)</term><term>Mutation (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Plasmides (génétique)</term><term>Pression osmotique (MeSH)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Transporteurs de cations (génétique)</term><term>Transporteurs de cations (métabolisme)</term><term>Triticum (génétique)</term><term>Triticum (métabolisme)</term><term>Vacuolar Proton-Translocating ATPases (génétique)</term><term>Vacuolar Proton-Translocating ATPases (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cation Transport Proteins</term><term>DNA, Complementary</term><term>DNA, Plant</term><term>Inorganic Pyrophosphatase</term><term>Plant Proteins</term><term>Sodium-Hydrogen Exchangers</term><term>Vacuolar Proton-Translocating ATPases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cation Transport Proteins</term><term>Inorganic Pyrophosphatase</term><term>Plant Proteins</term><term>Sodium-Hydrogen Exchangers</term><term>Vacuolar Proton-Translocating ATPases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plasmids</term><term>Saccharomyces cerevisiae</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>ADN des plantes</term><term>Antiport des ions sodium-hydrogène</term><term>Inorganic Pyrophosphatase</term><term>Plasmides</term><term>Protéines végétales</term><term>Saccharomyces cerevisiae</term><term>Transporteurs de cations</term><term>Triticum</term><term>Vacuolar Proton-Translocating ATPases</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antiport des ions sodium-hydrogène</term><term>Inorganic Pyrophosphatase</term><term>Protéines végétales</term><term>Transporteurs de cations</term><term>Triticum</term><term>Vacuolar Proton-Translocating ATPases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Gene Expression</term><term>Genes, Plant</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Osmotic Pressure</term><term>Phylogeny</term><term>Sequence Homology, Amino Acid</term><term>Sodium Chloride</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chlorure de sodium</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Expression des gènes</term><term>Gènes de plante</term><term>Mutation</term><term>Phylogenèse</term><term>Pression osmotique</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sodium at high millimolar levels in the cytoplasm is toxic to plant and yeast cells. Sequestration of Na+ ions into the vacuole through the action of tonoplast proton pumps (an H+ -ATPase in the case of yeast, and either a H+ -pyrophosphatase (H+ -PPase) or H+ -ATPase in the case of plants) and a Na+/H+ antiporter is one mechanism that confers salt tolerance to these organisms. The cloning and characterization of genes encoding these tonoplast transport proteins from crop plants may contribute to our understanding of how to enhance crop plant response to saline stress. We cloned wheat orthologs of the Arabidopsis genes AtNHX1 and AVP1 using the polymerase chain reaction and primers corresponding to conserved regions of the respective coding sequences, and a wheat cDNA library as template. The wheat NHX cDNA cloned by this approach was a variant of the previously reported TNHX1 gene. The vacuolar H+ -PPase pump we cloned (TVP1) is the first member of this gene family cloned from wheat; it is deduced translation product is homologous to proteins encoded by genes in barley, rice, and Arabidopsis. Function of TNHX1 as a cation/proton antiporter was demonstrated using the nhx1 yeast mutant. TNHX1 was capable of suppressing the hyg sensitivity of nhx1. Functional characterization of the wheat H+ -PPase TVP1 was demonstrated using the yeast ena1 (plasma membrane Na+ -efflux transporter) mutant. Expression of TVP1 in ena1 suppressed its Na+ hypersensitivity. Expression analysis of salt-stressed wheat plants showed substantial up-regulation of TNHX1 transcript levels as compared to control plants, while transcript accumulation for TVP1 was not greatly affected by exposure of plants to salt stress.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Berkowitz, Gerald A" sort="Berkowitz, Gerald A" uniqKey="Berkowitz G" first="Gerald A" last="Berkowitz">Gerald A. Berkowitz</name><name sortKey="Gaxiola, Roberto A" sort="Gaxiola, Roberto A" uniqKey="Gaxiola R" first="Roberto A" last="Gaxiola">Roberto A. Gaxiola</name><name sortKey="Masmoudi, Khaled" sort="Masmoudi, Khaled" uniqKey="Masmoudi K" first="Khaled" last="Masmoudi">Khaled Masmoudi</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Brini, Faical" sort="Brini, Faical" uniqKey="Brini F" first="Faïçal" last="Brini">Faïçal Brini</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/MaghrebDataLibMedV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000021 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000021 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= MaghrebDataLibMedV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:15907686
|texte= Cloning and characterization of a wheat vacuolar cation/proton antiporter and pyrophosphatase proton pump.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15907686" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MaghrebDataLibMedV2
| This area was generated with Dilib version V0.6.38. |  |